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Addgene

pMCSG7-10xHis-ecRNHI*
(Plasmid #219523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMCSG7
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cysteine-free variant of the E. coli ribonuclease HI gene
  • Species
    Escherichia coli
  • Mutation
    Cysteine free variant
  • Tag / Fusion Protein
    • N terminal 10xHis Tag

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMCSG7-10xHis-ecRNHI* was a gift from Arthur Palmer (Addgene plasmid # 219523 ; http://n2t.net/addgene:219523 ; RRID:Addgene_219523)

  • For your References section:

    Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers. Bhattacharya S, Varney KM, Dahmane T, Johnson BA, Weber DJ, Palmer AG 3rd. J Biomol NMR. 2024 Jun 10. doi: 10.1007/s10858-024-00443-w. 10.1007/s10858-024-00443-w PubMed 38856928
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