pMCSG7-10xHis-ecRNHI*
(Plasmid
#219523)
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Purposebacterial expression of cysteine-free variant of the E. coli ribonuclease HI gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219523 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMCSG7
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecysteine-free variant of the E. coli ribonuclease HI gene
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SpeciesEscherichia coli
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MutationCysteine free variant
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Tag
/ Fusion Protein
- N terminal 10xHis Tag
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCSG7-10xHis-ecRNHI* was a gift from Arthur Palmer (Addgene plasmid # 219523 ; http://n2t.net/addgene:219523 ; RRID:Addgene_219523)