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Addgene

SCP2-pUC119
(Plasmid #21951)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21951 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC119
  • Backbone size w/o insert (bp) 3162
  • Vector type
    a pUC derivative - a cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SCP2 (super core promoter 2)-pUC119
  • Species
    H. sapiens (human), D. melanogaster (fly); viral (CMV)
  • Insert Size (bp)
    81

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer M13 forward or M13/pUC sequencing primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several mismatches between depositor's sequence and Addgene sequence. The mismatches are mostly in vector and should not affect the insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SCP2-pUC119 was a gift from James Kadonaga (Addgene plasmid # 21951 ; http://n2t.net/addgene:21951 ; RRID:Addgene_21951)

  • For your References section:

    Rational design of a super core promoter that enhances gene expression. Juven-Gershon T, Cheng S, Kadonaga JT. Nat Methods. 2006 Nov . 3(11):917-22. 10.1038/nmeth937 PubMed 17124735
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