pAGD335
(Plasmid #219499)

• Purpose: pAGD248 derivative with the retron Eco1 msr, sfGFP flanked by 2 BsaI sites , and the rest of the retron recombineering operon (Eco1 RT, CspRect, MutL-E32K) downstream of the pBad promoter
• Depositing Lab: Seth Shipman
• Publication: Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.17.549397. doi: 10.1101/2023.07.17.549397.

Backbone

• Vector backbone: pKD46
• Backbone size w/o insert (bp): 6329
• Total vector size (bp): 9215
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Eco1 msr, sfGFP, Eco1 RT, CspRecT, MutLE32K
• Species: Synthetic
• Promoter: pBAD

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBAD
• 3′ sequencing primer: Lambda Terminator

Resource Information

• Supplemental Documents:
  • pAGD335.gb
• A portion of this plasmid was derived from a plasmid made by: Used Addgene items #114454 (pZS157) and #41583 (pCAGGS-mCherry).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.07.17.549397v1 for bioRxiv preprint.