pAGD236
(Plasmid #219497)

• Purpose: pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter
• Depositing Lab: Seth Shipman
• Publication: Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.17.549397. doi: 10.1101/2023.07.17.549397.

Backbone

• Vector backbone: pCDFduet-1
• Backbone manufacturer: Novagen (EMD Millipore)
• Backbone size w/o insert (bp): 3781
• Total vector size (bp): 5648
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sfGFP, Eco1 msr, Eco1 RT
• Species: Escherichia coli
• Insert Size (bp): 2247
• Promoter: T7

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-Term

Resource Information

• Supplemental Documents:
  • pAGD236.gb
• A portion of this plasmid was derived from a plasmid made by: Used Addgene items #114454 (pZS157) and #41583 (pCAGGS-mCherry).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.07.17.549397v1 for bioRxiv preprint.