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pCIG2-IRES-Lamp1-mNeon Green
(Plasmid #219441)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219441 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCIG2
  • Backbone size w/o insert (bp) 7450
  • Total vector size (bp) 9379
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LAMP1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1932
  • GenBank ID
    NM_012857.1
  • Entrez Gene
    Lamp1 (a.k.a. LGP120)
  • Promoter CAG
  • Tag / Fusion Protein
    • mNeonGreen (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Jayne Aiken, as described in Aiken et al., Human Molecular Genetics (2019)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIG2-IRES-Lamp1-mNeon Green was a gift from Erika Holzbaur (Addgene plasmid # 219441 ; http://n2t.net/addgene:219441 ; RRID:Addgene_219441)

  • For your References section:

    A RAB7A phosphoswitch coordinates Rubicon Homology protein regulation of Parkin-dependent mitophagy. Tudorica DA, Basak B, Puerta Cordova AS, Khuu G, Rose K, Lazarou M, Holzbaur ELF, Hurley JH. J Cell Biol. 2024 Jul 1;223(7):e202309015. doi: 10.1083/jcb.202309015. Epub 2024 May 10. 10.1083/jcb.202309015 PubMed 38728007
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