pHTN-LC3B
(Plasmid #219440)

• Purpose: Expresses HaloTag-LC3B, an autophagosome marker
• Depositing Lab: Erika Holzbaur
• Publication: Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e202309015. doi: 10.1083/jcb.202309015. Epub 2024 May 10.

Backbone

• Vector backbone: pHTN
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 6020
• Total vector size (bp): 6485
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LC3B
• Species: R. norvegicus (rat)
• Insert Size (bp): 462
• Entrez Gene: Map1lc3b (a.k.a. LC3B, Map1lc3, Mpl3, zbs559)
• Promoter: CMV
• Tag / Fusion Protein:
  • HaloTag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho I (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: pdHT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We constructed this plasmid using an insert encoding LC3B subcloned from a plasmid generously provided by Kabeya et al. EMBO J (2000)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry