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Addgene

pDonor-LoxP
(Plasmid #219436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219436 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMW118
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    two transposon ends, LoxP-terminator-LoxP gene cassette
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonor-LoxP was a gift from Sheng Yang (Addgene plasmid # 219436 ; http://n2t.net/addgene:219436 ; RRID:Addgene_219436)

  • For your References section:

    Chromosome recombination and modification by LoxP-mediated evolution in Vibrio natriegens using CRISPR-associated transposases. Xu J, Sun Y, Wu J, Yang S, Yang L. Biotechnol Bioeng. 2024 Mar;121(3):1163-1172. doi: 10.1002/bit.28639. Epub 2023 Dec 22. 10.1002/bit.28639 PubMed 38131162
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