pSIBR4-Cre
(Plasmid
#219435)
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PurposeTheophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219435 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSU2718
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameintron 4-Cre
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Speciesother
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIBR4-Cre was a gift from Sheng Yang (Addgene plasmid # 219435 ; http://n2t.net/addgene:219435 ; RRID:Addgene_219435) -
For your References section:
Chromosome recombination and modification by LoxP-mediated evolution in Vibrio natriegens using CRISPR-associated transposases. Xu J, Sun Y, Wu J, Yang S, Yang L. Biotechnol Bioeng. 2024 Mar;121(3):1163-1172. doi: 10.1002/bit.28639. Epub 2023 Dec 22. 10.1002/bit.28639 PubMed 38131162