pAAV_S5E2_Chrimson_tdTomato
(Plasmid
#219432)
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PurposeAAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-S5E2-dTom-nlsdTom
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameChrimson-tdTomato
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The PV-targeting regulatory element was originally described in Vormstein-Schneider et al, Nat Neurosci, 2020. The AAV vector was used in functional study in the following article: "Cortical reactivations predict future sensory responses." (PMID: 38093002; DOI: 10.1038/s41586-023-06810-1)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_S5E2_Chrimson_tdTomato was a gift from Gordon Fishell (Addgene plasmid # 219432 ; http://n2t.net/addgene:219432 ; RRID:Addgene_219432) -
For your References section:
Viral manipulation of functionally distinct interneurons in mice, non-human primates and humans. Vormstein-Schneider D, Lin JD, Pelkey KA, Chittajallu R, Guo B, Arias-Garcia MA, Allaway K, Sakopoulos S, Schneider G, Stevenson O, Vergara J, Sharma J, Zhang Q, Franken TP, Smith J, Ibrahim LA, M Astro KJ, Sabri E, Huang S, Favuzzi E, Burbridge T, Xu Q, Guo L, Vogel I, Sanchez V, Saldi GA, Gorissen BL, Yuan X, Zaghloul KA, Devinsky O, Sabatini BL, Batista-Brito R, Reynolds J, Feng G, Fu Z, McBain CJ, Fishell G, Dimidschstein J. Nat Neurosci. 2020 Dec;23(12):1629-1636. doi: 10.1038/s41593-020-0692-9. Epub 2020 Aug 17. 10.1038/s41593-020-0692-9 PubMed 32807948