GAL4-BRD4
(Plasmid
#21943)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSG424
- Backbone size w/o insert (bp) 3396
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBRD4
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SpeciesH. sapiens (human)
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Insert Size (bp)460
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Mutationaa 1209-1362
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Entrez GeneBRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
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Tags
/ Fusion Proteins
- Flag (N terminal on insert)
- GAL4-DNA-BD (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer Gal4-Nterm
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GAL4-BRD4 was a gift from Eric Verdin (Addgene plasmid # 21943 ; http://n2t.net/addgene:21943 ; RRID:Addgene_21943) -
For your References section:
Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription. Bisgrove DA, Mahmoudi T, Henklein P, Verdin E. Proc Natl Acad Sci U S A. 2007 Aug 21. 104(34):13690-5. 10.1073/pnas.0705053104 PubMed 17690245