pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine)
(Plasmid
#219426)
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PurposeCre-dependent expression of luciferase opsin fusion protein for bioluminescent optogenetics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219426 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 5336
- Total vector size (bp) 7613
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemNeonGreen, ISN-IE, ChRmine
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Alt nameLMO11
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SpeciesSynthetic; Oplophorus
- Promoter EF-1alpha
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Tag
/ Fusion Protein
- Gaussia luciferase signal sequence (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV EFIa DIO LMO11 (GeNL_SS-ChRmine) was a gift from Ute Hochgeschwender (Addgene plasmid # 219426 ; http://n2t.net/addgene:219426 ; RRID:Addgene_219426) -
For your References section:
Presymptomatic targeted circuit manipulation for ameliorating Huntington's disease pathogenesis. Ikefuama EC, Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender U. iScience. 2025 Feb 13;28(3):112022. doi: 10.1016/j.isci.2025.112022. eCollection 2025 Mar 21. 10.1016/j.isci.2025.112022 PubMed 40092615