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Addgene

pET28-a(+)-6xHis-miRFP(-5)-HO1
(Plasmid #219424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219424 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    miRFP682
  • Alt name
    monomeric near-infrared fluorescent protein 682
  • Mutation
    Contains Ribosomal Binding Site for expression of both miRFP682 and HO-1
  • Promoter T7
  • Tag / Fusion Protein
    • MGHHHHHGG (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    HO1
  • Alt name
    Heme Oxygenase 1
  • Promoter T7

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-a(+)-6xHis-miRFP(-5)-HO1 was a gift from Allie Obermeyer (Addgene plasmid # 219424 ; http://n2t.net/addgene:219424 ; RRID:Addgene_219424)

  • For your References section:

    Selectivity of Complex Coacervation in Multiprotein Mixtures. Ahn SY, Obermeyer AC. JACS Au, 2024 10.1021/jacsau.4c00399
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