pcDNA3.1/V5-His-mPer1
(Plasmid
#219421)
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PurposeExpresses 3873 bp of mPer1 coding sequence in E. coli.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1/V5-His
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 9342
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Vector typeMammalian Expression, Bacterial Expression
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Selectable markersNeomycin (select with G418), Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemPer1
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Alt nameMouse period circadian protein homolog 1
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SpeciesM. musculus (mouse)
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Insert Size (bp)3914
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Entrez GenePer1 (a.k.a. Hftm, Per, m-rigui, mPer1)
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- His (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1/V5-His-mPer1 was a gift from Aziz Sancar (Addgene plasmid # 219421 ; http://n2t.net/addgene:219421 ; RRID:Addgene_219421) -
For your References section:
In vitro DNA repair genomics using XR-seq with Escherichia coli and mammalian cell-free extracts. Cao X, Kose C, Selby CP, Sancar A. Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2314233120. doi: 10.1073/pnas.2314233120. Epub 2023 Oct 16. 10.1073/pnas.2314233120 PubMed 37844222