Skip to main content
Addgene

pcDNA3.1/V5-His-mPer1
(Plasmid #219421)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219421 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1/V5-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 9342
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418), Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mPer1
  • Alt name
    Mouse period circadian protein homolog 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3914
  • Entrez Gene
    Per1 (a.k.a. Hftm, Per, m-rigui, mPer1)
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • His (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1/V5-His-mPer1 was a gift from Aziz Sancar (Addgene plasmid # 219421 ; http://n2t.net/addgene:219421 ; RRID:Addgene_219421)
  • For your References section:

    In vitro DNA repair genomics using XR-seq with Escherichia coli and mammalian cell-free extracts. Cao X, Kose C, Selby CP, Sancar A. Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2314233120. doi: 10.1073/pnas.2314233120. Epub 2023 Oct 16. 10.1073/pnas.2314233120 PubMed 37844222