pFlag-CMV2-Brd4 FEE-AAA
(Plasmid #21942)

• Depositing Lab: Eric Verdin
• Publication: Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 104(34):13690-5.

Backbone

• Vector backbone: pFLAG-CMV2
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BRD4
• Species: H. sapiens (human)
• Insert Size (bp): 460
• Mutation: aa 1209-1362 only, FEE-AAA
• Entrez Gene: BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: inverse PCR (unknown if destroyed)
• 3′ cloning site: inverse PCR (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry