pET-6xHis-GFP(-7)-neg_tag1
(Plasmid
#219419)
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PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETDuet
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP(-7)-neg_tag1
- Promoter T7
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Tags
/ Fusion Proteins
- MGHHHHHGG (N terminal on insert)
- DEEEDD (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer TAATACGACTCACTATAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-6xHis-GFP(-7)-neg_tag1 was a gift from Allie Obermeyer (Addgene plasmid # 219419 ; http://n2t.net/addgene:219419 ; RRID:Addgene_219419) -
For your References section:
Selectivity of Complex Coacervation in Multiprotein Mixtures. Ahn SY, Obermeyer AC. JACS Au, 2024 10.1021/jacsau.4c00399