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Addgene

pET-6xHis-GFP(-7)-neg_tag1
(Plasmid #219419)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219419 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pETDuet
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP(-7)-neg_tag1
  • Promoter T7
  • Tags / Fusion Proteins
    • MGHHHHHGG (N terminal on insert)
    • DEEEDD (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-6xHis-GFP(-7)-neg_tag1 was a gift from Allie Obermeyer (Addgene plasmid # 219419 ; http://n2t.net/addgene:219419 ; RRID:Addgene_219419)
  • For your References section:

    Selectivity of Complex Coacervation in Multiprotein Mixtures. Ahn SY, Obermeyer AC. JACS Au, 2024 10.1021/jacsau.4c00399