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pET28-a(+)-6xHis-mBlueberry(-9)
(Plasmid #219416)

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  • Purpose
    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate
  • Depositing Lab
    Allie Obermeyer
  • Publication
    Ahn et al JACS Au, 2024 ( How to cite )
  • Sequence Information
    • Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219416 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mBlueberry2(-9)
  • Mutation
    K137E, K199E, R234E
  • Promoter T7
  • Tag / Fusion Protein
    • MGHHHHHGG (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-a(+)-6xHis-mBlueberry(-9) was a gift from Allie Obermeyer (Addgene plasmid # 219416 ; http://n2t.net/addgene:219416 ; RRID:Addgene_219416)

  • For your References section:

    Selectivity of Complex Coacervation in Multiprotein Mixtures. Ahn SY, Obermeyer AC. JACS Au, 2024 10.1021/jacsau.4c00399
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