pET28-a(+)-6xHis-LSSmCherry1(-13)
(Plasmid
#219415)
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PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET-28a(+)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLSSmCherry1(-13)
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MutationK137E, K199E
- Promoter T7
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Tag
/ Fusion Protein
- MGHHHHHGG (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TAATACGACTCACTATAG (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28-a(+)-6xHis-LSSmCherry1(-13) was a gift from Allie Obermeyer (Addgene plasmid # 219415 ; http://n2t.net/addgene:219415 ; RRID:Addgene_219415) -
For your References section:
Selectivity of Complex Coacervation in Multiprotein Mixtures. Ahn SY, Obermeyer AC. JACS Au, 2024 10.1021/jacsau.4c00399
