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Addgene

pKL2-138
(Plasmid #219413)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219413 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    HO Locus Integrating Vector
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Four Arg to Ala mutations in zinc finger backbone

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid encodes multiple transcriptional units.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKL2-138 was a gift from Ahmad Khalil (Addgene plasmid # 219413 ; http://n2t.net/addgene:219413 ; RRID:Addgene_219413)

  • For your References section:

    Cooperative assembly confers regulatory specificity and long-term genetic circuit stability. Bragdon MDJ, Patel N, Chuang J, Levien E, Bashor CJ, Khalil AS. Cell. 2023 Aug 31;186(18):3810-3825.e18. doi: 10.1016/j.cell.2023.07.012. Epub 2023 Aug 7. 10.1016/j.cell.2023.07.012 PubMed 37552983
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