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Addgene

pKL1-222
(Plasmid #219409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    LEU2 Integrating Vector
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
  • Species
    S. cerevisiae (budding yeast)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKL1-222 was a gift from Ahmad Khalil (Addgene plasmid # 219409 ; http://n2t.net/addgene:219409 ; RRID:Addgene_219409)

  • For your References section:

    Cooperative assembly confers regulatory specificity and long-term genetic circuit stability. Bragdon MDJ, Patel N, Chuang J, Levien E, Bashor CJ, Khalil AS. Cell. 2023 Aug 31;186(18):3810-3825.e18. doi: 10.1016/j.cell.2023.07.012. Epub 2023 Aug 7. 10.1016/j.cell.2023.07.012 PubMed 37552983
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