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pET22b-Empty-cleavableELP
(Plasmid #219401)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219401 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET22b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Empty-cleavableELP
  • Species
    Synthetic
  • Tag / Fusion Protein
    • 6xHis (C terminal on backbone)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning stie: NdeI (not destroyed), 3' cloning site: XhoI (not destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b-Empty-cleavableELP was a gift from Danielle Mai (Addgene plasmid # 219401 ; http://n2t.net/addgene:219401 ; RRID:Addgene_219401)

  • For your References section:

    Protease-Driven Phase Separation of Elastin-Like Polypeptides. Wirtz BM, Yun AG, Wick C, Gao XJ, Mai DJ. Biomacromolecules. 2024 Jul 9. doi: 10.1021/acs.biomac.4c00346. 10.1021/acs.biomac.4c00346 PubMed 38980747
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