pFlag-CMV2-Brd4 R1337P
(Plasmid
#21939)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFLAG-CMV2
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBRD4
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SpeciesH. sapiens (human)
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Insert Size (bp)460
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Mutationaa 1209-1362 only, R1337P
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Entrez GeneBRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site inverse PCR (unknown if destroyed)
- 3′ cloning site inverse PCR (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFlag-CMV2-Brd4 R1337P was a gift from Eric Verdin (Addgene plasmid # 21939 ; http://n2t.net/addgene:21939 ; RRID:Addgene_21939) -
For your References section:
Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription. Bisgrove DA, Mahmoudi T, Henklein P, Verdin E. Proc Natl Acad Sci U S A. 2007 Aug 21. 104(34):13690-5. 10.1073/pnas.0705053104 PubMed 17690245