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Addgene

Pinco-mNrp1dCy
(Plasmid #21936)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Pinco
  • Backbone manufacturer
    Pelicci's laboratory
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    neuropilin 1
  • Alt name
    Nrp; NP-1; Npn1; NPN-1; C530029I03; Nrp1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2749
  • Mutation
    deleted amino acids 884-923
  • GenBank ID
    NM_008737
  • Entrez Gene
    Nrp1 (a.k.a. C530029I03, NP-1, NPN-1, Npn1, Nrp)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Nrp1 cDNA was a gift from Dr. Andreas Püschel, Institut für Allgemeine Zoologie und Genetik Westfälische Wilhelms-Universität Münster

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pinco-mNrp1dCy was a gift from Guido Serini (Addgene plasmid # 21936 ; http://n2t.net/addgene:21936 ; RRID:Addgene_21936)

  • For your References section:

    Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. Valdembri D, Caswell PT, Anderson KI, Schwarz JP, Koenig I, Astanina E, Caccavari F, Norman JC, Humphries MJ, Bussolino F, Serini G. PLoS Biol. 2009 Jan 27. 7(1):e25. 10.1371/journal.pbio.1000025 PubMed 19175293
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