pGBMCS-SortA
(Plasmid #21931)

• Depositing Lab: Fuyuhiko Inagaki
• Publication: Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Backbone

• Vector backbone: pET21d
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL-21(DE3) and its derivatives can be used for protein expression.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sortase
• Alt name: srtA
• Alt name: sortA
• Alt name: sortase A
• Species: Staphylococcus aureus (strain N315)
• Insert Size (bp): 627
• Mutation: deleted amino acids 1-59
• GenBank ID: SA2316
• Entrez Gene: srtA
• Tags / Fusion Proteins:
  • GB1 (N terminal on insert)
  • His-tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We obtained srtA gene from NBRC and cloned it into expression vector.
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry