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Addgene

pGBHPS-SortA
(Plasmid #21930)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21930 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pET21
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL-21(DE3) and its derivatives can be used for protein expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sortase
  • Alt name
    srtA
  • Alt name
    sortA
  • Alt name
    sortase A
  • Species
    Staphylococcus aureus (strain N315)
  • Insert Size (bp)
    672
  • Mutation
    deleted amino acids 1-59
  • Tags / Fusion Proteins
    • GB1 (N terminal on insert)
    • His (N terminal on insert)
    • HRV3C cleagage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We obtained srtA gene from NBRC and cloned it into expression vector.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBHPS-SortA was a gift from Fuyuhiko Inagaki (Addgene plasmid # 21930 ; http://n2t.net/addgene:21930 ; RRID:Addgene_21930)
  • For your References section:

    Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method. Kobashigawa Y, Kumeta H, Ogura K, Inagaki F. J Biomol NMR. 2009 Mar . 43(3):145-50. 10.1007/s10858-008-9296-5 PubMed 19140010