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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21930 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21
- Backbone size w/o insert (bp) 5500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsBL-21(DE3) and its derivatives can be used for protein expression.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesortase
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Alt namesrtA
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Alt namesortA
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Alt namesortase A
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SpeciesStaphylococcus aureus (strain N315)
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Insert Size (bp)672
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Mutationdeleted amino acids 1-59
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Tags
/ Fusion Proteins
- GB1 (N terminal on insert)
- His (N terminal on insert)
- HRV3C cleagage site (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe obtained srtA gene from NBRC and cloned it into expression vector.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBHPS-SortA was a gift from Fuyuhiko Inagaki (Addgene plasmid # 21930 ; http://n2t.net/addgene:21930 ; RRID:Addgene_21930) -
For your References section:
Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method. Kobashigawa Y, Kumeta H, Ogura K, Inagaki F. J Biomol NMR. 2009 Mar . 43(3):145-50. 10.1007/s10858-008-9296-5 PubMed 19140010