pTracer-EF-mFxh
(Plasmid
#21927)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTracer EF-V5His
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5933
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFxh
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Alt nameRBM9, fox-2
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SpeciesM. musculus (mouse)
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Insert Size (bp)1131
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GenBank IDAF387322
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Entrez GeneRbfox2 (a.k.a. Fbm2, Fxh, Hrnbp2, Rbm9)
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Tags
/ Fusion Proteins
- V5 (C terminal on backbone)
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTracer-EF-mFxh was a gift from Mariano Garcia-Blanco (Addgene plasmid # 21927 ; http://n2t.net/addgene:21927 ; RRID:Addgene_21927) -
For your References section:
Fox-2 mediates epithelial cell-specific fibroblast growth factor receptor 2 exon choice. Baraniak AP, Chen JR, Garcia-Blanco MA. Mol Cell Biol. 2006 Feb . 26(4):1209-22. 10.1128/MCB.26.4.1209-1222.2006 PubMed 16449636