p3x-FLAG-hnRNPF
(Plasmid #21926)

• Depositing Lab: Mariano Garcia-Blanco
• Publication: Mauger et al Mol Cell Biol. 2008 Sep . 28(17):5403-19.

Backbone

• Vector backbone: p3xFLAG-CMV-7.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: hnRNPF
• Species: H. sapiens (human)
• Insert Size (bp): 1248
• GenBank ID: NP_001091674
• Entrez Gene: HNRNPF (a.k.a. HNRPF, OK/SW-cl.23, mcs94-1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3 Flags (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry