pEF-BOS-T7-CA150
(Plasmid
#21924)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEF-BOS
- Backbone size w/o insert (bp) 5300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCA150
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Alt nameTCERG-1
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SpeciesH. sapiens (human)
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Insert Size (bp)3200
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Entrez GeneTCERG1 (a.k.a. CA150, TAF2S, Urn1)
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Tag
/ Fusion Protein
- T7 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF-BOS-T7-CA150 was a gift from Mariano Garcia-Blanco (Addgene plasmid # 21924 ; http://n2t.net/addgene:21924 ; RRID:Addgene_21924) -
For your References section:
Transcriptional cofactor CA150 regulates RNA polymerase II elongation in a TATA-box-dependent manner. Sune C, Garcia-Blanco MA. Mol Cell Biol. 1999 Jul . 19(7):4719-28. PubMed 10373521