MuHKII-pGFPN3
(Plasmid #21922)

• Depositing Lab: Hossein Ardehali
• Publication: Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Backbone

• Vector backbone: pGFP-N3
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha, HB101, and other general purpose strains.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mutant huamn HKII (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3
• Species: H. sapiens (human)
• Insert Size (bp): 2751
• Mutation: This is the full length human HKII cDNA sequence with C->A (D209A and D657A) mutations in the catalytic sites of the enzyme in the N- and C-halves. The cDNA is cloned into pGFP-N3
• GenBank ID: NM_000189
• Entrez Gene: HK2 (a.k.a. HKII, HXK2)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: NA

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry