MuHKI-pGFPN3
(Plasmid #21919)

• Depositing Lab: Hossein Ardehali
• Publication: Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Backbone

• Vector backbone: pGFP-N3
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha, HB101, and other general purpose strains.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3
• Species: H. sapiens (human)
• Insert Size (bp): 2751
• Mutation: This is the full length human HKI cDNA sequence with a C->A (D657A) mutation in the catalytic site of the enzyme in the C-half. The cDNA is cloned into pGFP-N3
• GenBank ID: M75126
• Entrez Gene: HK1 (a.k.a. HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, NMSR, RP79, hexokinase)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: NA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry