TrHKI-pGFPN3
(Plasmid #21918)

• Depositing Lab: Hossein Ardehali
• Publication: Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Backbone

• Vector backbone: pGF-N3
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha, HB101 and other general purpose strains
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Truncated human HKI (lacking exon 1 sequence that encodes for the mitochondrial binding domain) in pGFP-N3 plasmid
• Species: H. sapiens (human)
• Insert Size (bp): 2688
• Mutation: This is the truncated human HKI cDNA (lacking exon 1 that encodes for the mitochondrial binding domain of HKI) in pGFP-N3
• GenBank ID: M75126
• Entrez Gene: HK1 (a.k.a. HK, HK1-ta, HK1-tb, HK1-tc, HKD, HKI, HMSNR, HXK1, NEDVIBA, NMSR, RP79, hexokinase)
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry