SFFV-TCF3-Brd 213
(Plasmid
#219164)
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PurposeTranscription factor TCF3 with a specific barcode assigned.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneSFFV-MCS
- Backbone size w/o insert (bp) 6611
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTCF3
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SpeciesH. sapiens (human)
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Insert Size (bp)1965
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MutationCodon optimized
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Entrez GeneTCF3 (a.k.a. AGM8, AGM8A, AGM8B, E2A, E47, ITF1, TCF-3, VDIR, bHLHb21, p75)
- Promoter The spleen focus-forming virus (SFFV) promoter
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Barcode sequence cloned into XbaI restriction site. Design of plasmid is as follows: SFFV-[TF sequence]-WPRE-[Barcode sequence].
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SFFV-TCF3-Brd 213 was a gift from Filipe Pereira (Addgene plasmid # 219164 ; http://n2t.net/addgene:219164 ; RRID:Addgene_219164)