9E10.ChWT.Gal4.pCDNA1/AMP
(Plasmid #21914)

• Depositing Lab: David Ron
• Publication: Wang et al Science. 1996 May 31. 272(5266):1347-9.

Backbone

• Vector backbone: pcDNA1.1/Amp
• Backbone size w/o insert (bp): 4767
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CHOP
• Species: M. musculus (mouse)
• Entrez Gene: Ddit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • GAL4-DNA-BD (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results show 6bp insertion in the sequencing adding 2 Gly residues immediately after the Myc tag.

Mammalian expression plasmid encoding wildtype mouse CHOP fused to DNA-binding domain of yeast Gal4.