CHOP 6: mCHOP-WT-9E10-pcDNA1
(Plasmid #21913)

• Depositing Lab: David Ron
• Publication: CHOP plasmids (unpublished)

Backbone

• Vector backbone: pcDNA1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MC1061/P3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHOP10
• Species: M. musculus (mouse)
• Insert Size (bp): 560
• Entrez Gene: Ddit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
• Tag / Fusion Protein:
  • 9E10 (Myc) (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

From depositing lab:
"9E10 (Myc-epitope) tagged mouse CHOP coding region only. The insert was joined to the epitope tag using patch-PCR and incorporating unique BamH1 and Xho1 sites for ligation into pcDNA1. Stocks are provided as MC1060 (or MC1061, we are not sure) host strain transformed with the plasmid. Note that pCDNA1 plasmids must be propagated on combined amp and tet selection. I would recommedn that you consider transferring the insert into a better host strain such as pcDNA3"