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Addgene

pAL119-Flt3L
(Plasmid #21910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAL119
  • Backbone size w/o insert (bp) 7300
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human Soluble Fms-like Tyrosine Kinase 3 Ligand
  • Alt name
    Flt3L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1200
  • GenBank ID
    BC136464
  • Entrez Gene
    FLT3LG (a.k.a. FL, FLG3L, FLT3L)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAL119-Flt3L was a gift from Maria Castro (Addgene plasmid # 21910 ; http://n2t.net/addgene:21910 ; RRID:Addgene_21910)

  • For your References section:

    Inflammatory and anti-glioma effects of an adenovirus expressing human soluble Fms-like tyrosine kinase 3 ligand (hsFlt3L): treatment with hsFlt3L inhibits intracranial glioma progression. Ali S, Curtin JF, Zirger JM, Xiong W, King GD, Barcia C, Liu C, Puntel M, Goverdhana S, Lowenstein PR, Castro MG. Mol Ther. 2004 Dec . 10(6):1071-84. 10.1016/j.ymthe.2004.08.025 PubMed 15564139
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