CHOP 4: Mouse CHOP-WT-9E10-pSRa
(Plasmid
#21903)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21903 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSRa
- Backbone size w/o insert (bp) 4534
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Vector typeRetroviral
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCHOP10
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SpeciesM. musculus (mouse)
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Insert Size (bp)605
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Entrez GeneDdit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
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Tag
/ Fusion Protein
- 9E10-myc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer Neo-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pSRa and its associated helper plasmids are described in: Muller, AJ et al 1992 Mol Cell Biol 11:941, and helper plasmids were obtained from the lab of Dr. Owen Witte
NB: From Addgene's sequencing results, there is a 6bp insertion into the insert of CHOP4 that is not in the depositor's sequence.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CHOP 4: Mouse CHOP-WT-9E10-pSRa was a gift from David Ron (Addgene plasmid # 21903 ; http://n2t.net/addgene:21903 ; RRID:Addgene_21903)