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Addgene

9E10.ChS78&81A.Gal4.pCDNA1
(Plasmid #21901)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21901 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA1.1/Amp
  • Backbone size w/o insert (bp) 4767
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CHOP
  • Species
    M. musculus (mouse)
  • Mutation
    S78A S81A
  • Entrez Gene
    Ddit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • GAL4-DNA-BD (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mammalian expression plasmid encoding S78A & S81A mutant mouse CHOP fused to DNA-binding domain of yeast Gal4.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    9E10.ChS78&81A.Gal4.pCDNA1 was a gift from David Ron (Addgene plasmid # 21901 ; http://n2t.net/addgene:21901 ; RRID:Addgene_21901)

  • For your References section:

    Stress-induced phosphorylation and activation of the transcription factor CHOP (GADD153) by p38 MAP Kinase. Wang XZ, Ron D. Science. 1996 May 31. 272(5266):1347-9. 10.1126/science.272.5266.1347 PubMed 8650547
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