CHOP::GFP
(Plasmid #21898)

• Depositing Lab: David Ron
• Publication: Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22.

Backbone

• Vector backbone: pGL3 and pGL2 modified
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4310
• Vector type: Non-viral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: Stbl2 30C
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CHOP
• Species: M. musculus (mouse)
• Insert Size (bp): 9300
• Entrez Gene: Ddit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: EBV-R
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This reporter gene closely tracks the expression of endogenous CHOP.