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Addgene

pET01-TAF5-ex8-Minigene-SRSF1 motif MUT
(Plasmid #218973)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218973 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET01
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
  • Species
    H. sapiens (human)
  • Mutation
    mutant SRSF1 binding motif (AGTCTCCT)
  • Promoter RSV LTR

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET01-TAF5-ex8-Minigene-SRSF1 motif MUT was a gift from Thomas Gonatopoulos-Pournatzis (Addgene plasmid # 218973 ; http://n2t.net/addgene:218973 ; RRID:Addgene_218973)

  • For your References section:

    Genome-scale exon perturbation screens uncover exons critical for cell fitness. Xiao M-S, Damodaran AP, Kumari B, Dickson E, Xing K, On TA, Parab N, King HE, Perez AR, Guiblet WM, Duncan G, Che A, Chari R, Andresson T, Vidigal JA, Weatheritt RJ, Aregger M, Gonatopoulos-Pournatzis T. Mol Cell. 2024 June 24. doi: 10.1016/j.molcel.2024.05.024. 10.1016/j.molcel.2024.05.024
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