pQE81XN-moxGFP2
(Plasmid
#218962)
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PurposeExpresses Hisx6-Tagged moxGFP2 in bacteria
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218962 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQE81XN
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemoxGFP2
- Promoter T5
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Tag
/ Fusion Protein
- 6X-His
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer AATAGATTCAATTGTGAGCGGATAACAATTTCA
- 3′ sequencing primer ACCCGGGGTACCGAGTGATCTAGA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQE81XN-moxGFP2 was a gift from Benjamin Glick (Addgene plasmid # 218962 ; http://n2t.net/addgene:218962 ; RRID:Addgene_218962)