sc AAV miR26a eGFP
(Plasmid #21894)

• Depositing Lab: Joshua Mendell
• Publication: Kota et al Cell. 2009 Jun 12. 137(6):1005-17.

Backbone

• Vector backbone: pEMBL
• Backbone size w/o insert (bp): 3952
• Vector type: Mammalian Expression, AAV ; sc

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EF1 alpha -miR26a GFP p(A)
• Insert Size (bp): 2099
• Mutation: N/A
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Spe I (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

miR-26a sequence is 5'-TTCAAGTAATCCAGGATAGGC

Addgene sequencing results confirm the presence of both FseI sites flanking miR-26a.