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pET28a-ImpBeta1-mEosEM
(Plasmid #218933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218933 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 8557
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Importin Beta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3348
  • Entrez Gene
    KPNB1 (a.k.a. IMB1, IPO1, IPOB, Impnb, NTF97)
  • Promoter T7
  • Tags / Fusion Proteins
    • mEos4b (EosEM) (C terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 promoter
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Importin Beta1 gene was provided by Dirk Görlich in plasmid pQE9-Beta1, which was first reported in Kutay, et al. (1997) EMBO J. 16:1153-1163. The mEosEM gene was a gift from P. Xu (Addgene ID#132708), which was first reported in Fu, et al. (2020) Nat. Meth. 17:55-58.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-ImpBeta1-mEosEM was a gift from Siegfried Musser (Addgene plasmid # 218933 ; http://n2t.net/addgene:218933 ; RRID:Addgene_218933)

  • For your References section:

    Overlapping nuclear import and export paths unveiled by two-colour MINFLUX. Sau A, Schnorrenberg S, Huang Z, Bandyopadhyay D, Sharma A, Gurth CM, Dave S, Musser SM. Nature. 2025 Mar 19. doi: 10.1038/s41586-025-08738-0. 10.1038/s41586-025-08738-0 PubMed 40108461
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