Dual-CRISPR-Cas9-U6-H1 (pBP48)
(Plasmid
#218932)
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Purpose(Empty Backbone) The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218932 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePX459
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Vector typeMammalian Expression, CRISPR
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Selectable markersPuromycin
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Tags
/ Fusion Proteins
- 3XHA (N terminal on insert)
- 2A-Puro (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- 5′ sequencing primer hU6-F
- 3′ sequencing primer hH1-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dual-CRISPR-Cas9-U6-H1 (pBP48) was a gift from Baoxu Pang (Addgene plasmid # 218932 ; http://n2t.net/addgene:218932 ; RRID:Addgene_218932) -
For your References section:
Genome-wide Cas9-mediated screening of essential non-coding regulatory elements via libraries of paired single-guide RNAs. Li Y, Tan M, Akkari-Henic A, Zhang L, Kip M, Sun S, Sepers JJ, Xu N, Ariyurek Y, Kloet SL, Davis RP, Mikkers H, Gruber JJ, Snyder MP, Li X, Pang B. Nat Biomed Eng. 2024 May 22. doi: 10.1038/s41551-024-01204-8. 10.1038/s41551-024-01204-8 PubMed 38778183
