pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
(Plasmid
#218916)
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PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218916 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV
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Backbone manufacturerVectorBuilder
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
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SpeciesOther
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR was a gift from Sven Eyckerman (Addgene plasmid # 218916 ; http://n2t.net/addgene:218916 ; RRID:Addgene_218916) -
For your References section:
Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics. Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, Jacobs T, Eyckerman S. 2024, Cell Reports Methods 4, 100818 10.1016/j.crmeth.2024.100818
