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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
(Plasmid #218916)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218916 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV
  • Backbone manufacturer
    VectorBuilder
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
  • Species
    Other

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR was a gift from Sven Eyckerman (Addgene plasmid # 218916 ; http://n2t.net/addgene:218916 ; RRID:Addgene_218916)

  • For your References section:

    Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics. Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, Jacobs T, Eyckerman S. Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9. 10.1016/j.crmeth.2024.100818 PubMed 38986614
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