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pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA
(Plasmid #218911)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218911 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    V5-TurboID-MUTT2A-huGR/BlastR
  • Species
    H. sapiens (human); Other
  • Mutation
    2363G>C and 4155C>T and 4729T>G and 4731T>G and 6412T>C
  • Entrez Gene
    NR3C1 (a.k.a. GCCR, GCR, GCRST, GR, GRL)

Cloning Information

  • Cloning method Golden Gate

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA was a gift from Sven Eyckerman (Addgene plasmid # 218911 ; http://n2t.net/addgene:218911 ; RRID:Addgene_218911)

  • For your References section:

    Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics. Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, Jacobs T, Eyckerman S. Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9. 10.1016/j.crmeth.2024.100818 PubMed 38986614
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