pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA
(Plasmid
#218910)
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PurposePiggyBac transposition
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218910 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMCS-AAT-PB:PGKpuro∆tk
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Backbone manufacturerWellcome Trust Sanger
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameV5-TurboID-T2A-huGR/BlastR
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SpeciesH. sapiens (human); Other
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Mutation2363G>C and 4155C>T and 4729T>G and 4731T>G and 6412T>C
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Entrez GeneNR3C1 (a.k.a. GCCR, GCR, GCRST, GR, GRL)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyA was a gift from Sven Eyckerman (Addgene plasmid # 218910 ; http://n2t.net/addgene:218910 ; RRID:Addgene_218910) -
For your References section:
Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics. Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, Jacobs T, Eyckerman S. Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9. 10.1016/j.crmeth.2024.100818 PubMed 38986614