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Addgene

pEN-X-hPGK-HygroR-Z
(Plasmid #218905)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218905 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTwist Kan High Copy
  • Backbone manufacturer
    Twist Biosciences
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPGK-HygroR
  • Species
    Other
  • Mutation
    246T>A and 398T>C

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.11.03.565112 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN-X-hPGK-HygroR-Z was a gift from Sven Eyckerman (Addgene plasmid # 218905 ; http://n2t.net/addgene:218905 ; RRID:Addgene_218905)
  • For your References section:

    Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics. Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, Jacobs T, Eyckerman S. Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9. 10.1016/j.crmeth.2024.100818 PubMed 38986614