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Addgene

pGP-RSET-iGABASnFR2(no bind)-6xHis
(Plasmid #218887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSET
  • Backbone manufacturer
    ThermoFisher Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iGABASnFR2(no bind)
  • Alt name
    variant 4444
  • Species
    Pseudomonas fluorescens, A. victoria (jellyfish)
  • Insert Size (bp)
    1710
  • Mutation
    S99A F102G R168P
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-RSET-iGABASnFR2(no bind)-6xHis was a gift from GENIE Project (Addgene plasmid # 218887 ; http://n2t.net/addgene:218887 ; RRID:Addgene_218887)
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