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pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10
(Plasmid #218867)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 218867 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJFRC59
  • Backbone manufacturer
    Gerald Rubin
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RSET-jGCaMP8f
  • Alt name
    variant 456
  • Species
    R. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)
  • Insert Size (bp)
    1353
  • Mutation
    Q315L
  • Promoter hsp70
  • Tag / Fusion Protein
    • 6xHis-T7 gene 10 leader-Xpress epitope-Enterokinase site (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

RSET leader improves expression and basal fluorescence compared to #162385

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10 was a gift from GENIE Project (Addgene plasmid # 218867 ; http://n2t.net/addgene:218867 ; RRID:Addgene_218867)

  • For your References section:

    jGCaMP8 Variants with Improved Expression for Drosophila. Reagents G, Hasseman JP, Turner GC, L Looger L, Bushey D, Reep D, Tsang A, Zheng J, Korff W, Jayaraman V, Kolb I, Zhang Y.. figshare Dataset. 2024. doi: 10.6084/m9.figshare.26231087.v1. 10.6084/m9.figshare.26231087.v1
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