Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP
(Plasmid
#218857)
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PurposeXla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218857 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneTol2 pDest
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehGCGR
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SpeciesH. sapiens (human)
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Entrez GeneGCGR (a.k.a. GGR, GL-R, MVAH)
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Tag
/ Fusion Protein
- QF fusion protein (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://pubmed.ncbi.nlm.nih.gov/37066422/ for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP was a gift from Marnie Halpern (Addgene plasmid # 218857 ; http://n2t.net/addgene:218857 ; RRID:Addgene_218857) -
For your References section:
Transsynaptic labeling and transcriptional control of zebrafish neural circuits. Coomer CE, Naumova D, Talay M, Zolyomi B, Snell NJ, Sorkaç A, Chanchu JM, Cheng J, Roman I, Li J, Robson D, McLean DL, Barnea G, Halpern ME. Nat Neurosci (2024). 10.1038/s41593-024-01815-z