N-SF-TAP-DEST-TLK2-D592V
(Plasmid #218835)

• Purpose: Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).
• Depositing Lab: Travis Stracker
• Publication: Identification of de novo mutations in TLK1 and MDM1 in a patient with a neurodevelopmental disorder and immunodeficiency (unpublished)

Backbone

• Vector backbone: modified pcDNA3.0
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 7567
• Modifications to backbone: Insertion of Gateway cloning cassette and affinity tags: https://doi.org/10.1002/pmic.200700038
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TLK2 Q592V (kinase dead)
• Species: H. sapiens (human)
• Insert Size (bp): 2251
• Mutation: Q592V
• GenBank ID: NM_006852.6 NM_001375271.1
• Entrez Gene: TLK2 (a.k.a. HsHPK, MRD57, PKU-ALPHA)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG-STREP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 Forward
• 3′ sequencing primer: SP6 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was constructed in part with THC shRNAs from the MISSION library (Merck).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry